What You Need to Know About Lateral Flow Readers

Lateral test refers to a special type of paper-based test that is made up of a device used for detecting the presence or absence of a target in a liquid without necessarily having to use complicated and costly equipment. The tests and the results are mostly conducted by specialized lab technicians. However, they can also be carried out both in medical diagnostics and in-home testing, laboratory or even point of care testing. A perfect example of lateral flow tests is the pregnancy test. You'll also want to check out  DOA Test Reader.

It is worth noting that the test runs on a series of capillary beds including porous paper and polymer. The polymer can either be microstructured or sintered. Every element has the ability to move the fluid test spontaneously thereby enabling the readers to note the changes in the flow of the liquid test and make inference about the phenomena. For one to use the lateral flow test, they need to first place the liquid in the first element on a sponge that holds the excess of the sample fluid. Once the fluid is placed on the sponge, it then migrates to the second element where the manufacture of the device has placed a dried format of bio-active particles also referred to as conjugate. The conjugate is essential as it contains most elements that guarantee optimized chemical reaction between the elements in the fluid and the chemical in the conjugate. You'll also want to learn more about how  GenPrime can help you out.

As the sample fluid dissolves with the conjugate, it also dissolves with the particles and it is later transported to the porous structure thereby making the analyte to bind to the particles while migrating further through the next stage of the test. It is worth noting that there are two main ways in which lateral flow tests can operate, these include competitive and sandwich assay. In the sandwich assay, as the sample fluid moves along the assay it has to first meet a conjugate labeled as antibodies to the specific target analyte. It is worth noting that the conjugate antibodies will bind when the analyte is detected within the sample, hence enabling them to reach the test line with the antibodies specific to the target.
The competitive assay, on the other hand, differs from the sandwich assay in that the former operates by first making the sample fluid to first interact with the colored particles which are labeled with the target analyte. In addition, the test line is made of antibodies to the target. On the other hand, the unlabeled analyte in the samples will bar the binding sites on the antibodies thereby preventing the acceptance of the colored particles. Here's the process of the lateral flow test:  https://youtu.be/9rfgwyQFC_0